Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. SpectraMax Multi-Mode Microplate Readers. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). 4. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. Other technologies such as imaging. Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. Values were determined according to a standard curve (log/log curve-fit). 00 USD. , Sunnyvale, CA). Awareness Technology, Inc. The cell survival. Page: 1/6. 5 mL of 1 M acetate buffer (pH 4. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. Awareness Technology, Inc. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. , Sunnyvale, CA, USA) at a wavelength of 450 nm. SpectraMax 384 Plus. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. G. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Service parts for these models are not being produced and are no longer in stock. Although all samples initially were analyzed at the same volume, the volume of sample. 1% N-1-naphtylethylenediamine dihydrochloride, 2. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Call 1-800-745-2710. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. The light features a slim profile to prevent. Description 1. Data System. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. If depot service is the best option for you we will assist you with logistics to help you keep. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 5. Near bottom. control. 06 ng/mL; FSH: 0. well, and strip-well microplates. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. A 0. 81. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Includes: SpectraMax 340PC384. 05 ng/mL; progesterone: 2. Values were determined according to a standard curve (log/log curve-fit). Water – distilled water or equivalent. Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. Figure 1. Each experiment was performed in duplicate and repeated at. Acceptor filter: 665/10 nm. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Microplate Readers. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. SpectraMax Gemini EM & FLEXstation (bottom read) 1. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). California Lightworks uses only the highest quality LEDs from Osram. Data were expressed as mean percentage of viable cells. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. These models can no longer be repaired. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax Multi-Mode Microplate Readers. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). The VersaMax and SpectraMax 190 read 96-well microplates. 00 USD. Molecular Device M2e. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Donor: 616/10 nm. TDP-43 stress granular cells were treated with 70% ice-cold. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The absorbances were being measured at 515 nm. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). The loading equaled the mass of BSA per mass of particles. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). 1x10 5 transduced, GFP + SK-NEP-1. edu Contact Phone: 216-368-8962. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Microplate Spectrophotometers. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. #N207) LAL Reagent Water (Lonza cat. SPECTRAmax® 190. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. SpectraMax Multi-Mode Microplate Readers. Molecular Devices SpectraMAX 340 Microplate Reader. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 305 reference level saturation. 30 µs. ), which was proportional to the number of damaged cells. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). , Sunnyvale, CA, USA) at 595 nm. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Immunofluorescence and Aggregation Analysis. Examples: “Press carefully to engage,” “Do not press down. Table 1. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). Softmax PRO v5. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Assay buffer was 50 m M Hepes, pH 7. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. SpectraMAX 340. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. The plate was covered and incubated for one hour at room temperature. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 003 OD Stray light ≤ 0. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). 6. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. Spectrophotometer (SpectraMax 340). PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. The absorbance at 405 nm was determined using a microplate reader (spectraMAX 340). Plasminogen was added to the fibrinogen before addition of thrombin and either tissue plasminogen activator (tPA) or urokinase plasminogen activator in the. VersaMax reads 96-well and strip-well plates. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Cross sections of mouse skin was prepared and stained as previously described . For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). As a common indicator of equine T cell activation, cytokine induction. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. Introduction. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. 340 nm / 80 nm. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. 313 reference gain check fail. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. , Sunnyvale, CA) at 490 nm. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. The SPECTRAmax 340PC can measure. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Data were expressed as the mean percent viable cell vs. Molecular Devices Spectramax 340 Plate Reader Repair. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Please see photos. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. 001/min at 425 nm. Details. Microscopy. Endpoint, kinetic, spectrum. 2. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. 1% PEG 8000 at 37°C. 0. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. They are for reference only. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. 30. Quick View. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Call 1-800-745-2710. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The ABS reader ranges from. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). Fully tested with Warranty. Six wells were assayed for each set of conditions and stadard deviations. ) at 490 nm. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. 6) and 0. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. The initial rate of hydrolysis was determined using the early linear region of. Calculating % of total mediator release in RBL assay. , Sunnyvale, CA, USA) at 595 nm. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. 5. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. When necessary, samples were. 0200-0729. Binding affinity for denosumab. Samples with OD values of less than 0. Bioz Stars score: 86/100, based on 1 PubMed citations. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. 3 mm. 5% phosphoric acid) in microplates at room temperature for 10 min. Product Keyword. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 1 0 100 80 60 40 20 0 4. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Service Contracts, Preventative Maintenance and Performance. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. No power cord or accessories included. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. 6. 0 2. SpectraMAX 340 plate reader (Molecular Devices). Each experiment was performed in duplicate. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 7. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). SpectraMax 190 reads 96-well and strip-well plates. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. One plate carrier adapter is provided with the instrument. reader (SpectraMax 340; Molecular devices, LLc). The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Customer Transformation Hub. Alves, Amelia K. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. Stat Fax 3200 Microplate Reader. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. Details. All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. One plate carrier adapter is provided with the instrument. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Pharmacokinetic and pharmacodynamic analysis. Call 1-800-745-2710. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. (Shiga, Japan) and kept for 30 min in the dark. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. Cell permeabilization. Data are expressed as percent of maximum binding for each protein. 5mM), and changes in OD 405 nm. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. Gerritsma,B. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. TDP-43 stress granular cells were treated with 70% ice-cold. Tunable for filterless flexibility. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. 20. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. 305 reference level saturation. 5. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). using SpectraMax i3 (Molecular devices, San Jose, USA). With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Analysis of ALA by Ehrlich’s Reagent Assay. SPECTRAmax® 190. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Alkaline phosphatase (ALP) activity. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. SoftMax Pro. 25–0. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Each SpectraMax® 340 PC 384 Microplate Reader has an. , Sunnyvale, CA). 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. 0200-6117. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. FlexStation® 3 Multi-Mode Microplate Reader. 024). Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. SpectraMax 250 With Computer SoftwareSpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. Molecular Devices SpectraMax Gemini XS Microplate Reader. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). 5 mL sample or standard solution was well mixed with 0. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). Rate of fibrinolysis. 30. These lab workhorses are used in drug discovery, research, bioassay validation, and. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. 5. Intra- and inter-assay coefficients of variation were lower than 8. The. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). (1988) and recorded at 412 nm. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. , Sunnyvale, CA). Stat Fax 4700 Microstrip Reader. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Both readers feature an 8-channel read head for fast reads and. This test was performed in duplicates. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. , Sunnyvale, Calif. 5. 0 to 4. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 1 mL of acetylacetone. Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. Statistical analysis. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. 303 unable to cal dark current. MOLECULAR DEVICE M5. To. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The SpectraMax® iD3 Multi Mode Microplate . TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. P. ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. 5 mL of 1 M acetate buffer (pH 4. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. 0. Products : SpectraMax Plus 384 reader,. edu Contact Phone: 216-368-8962. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. 5), L-ascorbic acid (25. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. Warranty. Molecular Devices Spectramax 340PC instructions. 6 g sodium acetate trihydrate and 2. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co.